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Microscopic magnificence

Life is dynamic. A big part of my scientific career is focused on recording the mechanisms of life. Below you'll find some microscopy videos of my research, mostly from my PhD.

Light-sheet microscopy

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Optical sectioning of C. elegans' anterior neuronswith home build light-sheet microscopy.

Movie of z-stack of neuronal microtubules, visualized with TBB-4::EGFP, imaged with light-sheet microscopy. From chapter 3 of my PhD thesis.

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Neuronal activity in the tail region of C. elegans

Neuronal activity visualized with GCaMP in the tail region, imaged with home build light-sheet microscope.
From chapter 3 of my PhD thesis.

Femto-second laser ablation

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Cutting off ciliary protein import

Fluorescence microscopy movie of pair of C. elegans' phasmid cilia (XBX-1::EGFP strain) pre and post-ablation. From chapter 4 of my PhD thesis.

Live single-molecule microscopy

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Single-molecule turnarounds of
intraflagellar transport
at the C. elegans ciliary tip

Tip turnarounds of OSM-6::EGFP in C. elegans phasmid cilia. Ciliary bases are located in the lower half of the video, ciliary tips in the upper half.

Transient accumulation of OSM-6::EGFP
at the ciliary tip

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Single-molecule imaging of the GPCR SRB-6 in C. elegans cilia

Single-particle fluorescence image sequences of endogenously labeled SRB-6, showing its low expression level in the phasmid cilia and mostly diffusive and salutatory motility.

Single-molecule imaging of calcium channel OCR-2

Collage of example single-molecule image sequences of OCR-2::EGFP, demonstrating the diversity in OCR-2 motility.

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Live-cell microscopy in Fungi

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Transport of lipids in a hyphae of symbiotic fungi

Live-cell fluorescence imaging of lipid dyes in symbiotic Arbuscular Mycorrhizal Fungi. From my post-doc research at AMOLF.

Transport of mitochondria in symbiotic fungi

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Live-cell microscopy of fluorescently labeled nuclei in fungi

Multi-directional transportation
in fungal hyphae

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Lepidoptera

During my internship in UC Berkely, I studied butterflies

Fluorescence microscopy image showing butterfly scales

With actin staining colored in red, which give rise to the structures that give color to the scales.

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Electron micropgraph of scales on a butterfly wing

Super-resolution microscopy

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STORM image of microtubules in young neuron

STochastic Optical Reconstruction Microscopy (STORM) image. Superposition of four images taken at different depths, showing the microtubules going around the nucleus.

STORM image of microtubules around centrosome

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