Supplemental movies to my PhD thesis Sensing Cilia

Chapter 3 - Imaging adult C. elegans live using light sheet microscopy

 

Movie S3.1: IFT dynamics.

IFT dynamics of OSM-6::EGFP, an IFT-B particle complex component, in the phasmid cilia of C. elegans, imaged with light-sheet microscopy. Related to Figure 2D.

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Movie S3.2: Head region neurons with light-sheet microscopy.

Movie of z-stack of  neuronal microtubules, visualized with TBB-4::EGFP, imaged with light-sheet microscopy. Related to Figure 3A.

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Movie S3.3: Head region neurons with epifluorescence microscopy.

Movie of z-stack of  neuronal microtubules, visualized with TBB-4::EGFP, imaged with epifluorescence microscopy. Related to Figure 3C

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Movie S3.4: Neuronal cell bodies in the head region.

3D visualization of z-stack of GCaMP, imaged with light-sheet microscopy. Related to Figure 4A.

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Movie S3.5: Neuronal activity in the tail region.

Neuronal activity visualized with GCaMP in the tail region, imaged with light-sheet microscopy. Related to Figure 4B-E.

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Chapter  4 - Cutting off ciliary protein import:

Intraflagellar transport after dendritic femtosecond-laser ablation

Movie S4.1: IFT-dynein redistribution and slow-down.

Fluorescence movie of pair of phasmid cilia (XBX-1::EGFP strain) pre and post-ablation. Time and scale bar are indicated. Same data as in Figure 4.2.

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Movie S4.2: IFT dynein and OSM-3 distribution and dynamics in C. elegans phasmid cilia before and after low-concentration azide treatment. Sodium azide is added at t = 0 s. Scale bar, 2 um. Video plays at 5x real time (time indicated). Video corresponds to Figure4.4A, third horizontal panel, and Supplemental Figure 4.2A, third horizontal panel.

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Chapter  5 - Single-molecule turnarounds of intraflagellar transport

at the C. elegans ciliary tip

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Movie S5.1: Single-Molecule Tip Turnarounds of OSM-6::EGFP in C. elegans Phasmid Cilia. Related to Figures 5.1 and 5.2. Ciliary bases are located in the lower half of the video, ciliary tips in the upper half.

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Movie S5.2: Transient Accumulation of OSM-6::EGFP at the Ciliary Tip. Related to Figure 5.1. Ciliary base is located in the lower half of the video, ciliary tip in the upper half.

 

Chapter  6 - OCR-2 is distributed along C. elegans chemosensory

cilia by diffusion in a local interplay with intraflagellar transport

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Movie S6.1: Redistribution of IFT-dynein upon repellent exposure. Fluorescence microscopy image sequence showing the reversible redistribution of IFT-dynein (XBX-1::EGFP) in the distal segments of the phasmid chemosensory cilia of C. elegans after the addition of a droplet of 5 µl 0.1% (W/V) SDS. Related to Figure 6.1.

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Movie S6.2: Single-molecule imaging of SRB-6.

Single-particle fluorescence image sequences of endogenously labeled SRB-6, showing its low expression level in the phasmid cilia and mostly diffusive and salutatory motility.

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Movie S6.3:Single-molecule imaging of OCR-2.

Collage of example single-molecule image sequences of OCR-2::EGFP, demonstrating the diversity in OCR-2 motility. Related to Figure 6.3A.